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KMID : 0604520190450040341
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Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 4 p.341 ~ p.351
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Anti-oxidative and Anti-bacterial Constituents from the Extracts of Rhododendron weyrichii Leaves
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Kim Jung-Eun
Jo Seong-Mi
Lee Nam-Ho
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Abstract
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In this study, we investigated anti-oxidative and anti-bacterial constituents from Rhododendron weyrichii leaves.
DPPH and ABTS+ radical scavenging activities were screened for the ethanol extract and solvent fractions. Potent scavenging activities were appeared from the extract, ethyl acetate (EtOAc) and n-butanol (BuOH) fractions. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, extract, n-hexane (Hex) and EtOAc fractions showed strong activities. To isolate the active constituents, the EtOAc fraction was further purified to afford five phytochemicals; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4) and isoquercetin (5). All of the compounds 1?5 were isolated for the first time from this plant. Among the isolates, the compound 4 and 5 showed strong DPPH and ABTS+ radical scavenging activities. Also, compound 3 exhibited the most potent anti-bacterial activity.
In addition, the content of astragalin isolated from this plant was determined by UPLC and the quantity was about 8.1 mg/g for the 70% ethanol extract and 34.8 mg/g for the EtOAc fraction. Based on these results, it is concluded that R. weyrichii extract could be potentially applicable as anti-oxidative and anti-bacterial ingredients in cosmetic formulations.
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KEYWORD
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Rhododendron weyrichii, anti-oxidation, anti-bacterial activity, isolation
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